Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk

Asparagus macrorrhizus: is a new species, which has been recently described. It is limited to the area surrounding the "Mar Menor" lagoon, in Murcia (Spain), and is the only "Critically Endangered" species of the genus Asparagus. Despite being protected, the number of plants has decreased in the last years due to the urbanization of its natural habitat. This species is a valuable genetic resource for asparagus breeding because of its special characteristics. So, the development of a micropropagation protocol is crucial to its conservation and use in breeding programs. The micropropagation protocol from asparagus rhizome buds previously developed by our research group has been adapted for A. macrorrhizus. Rhizome buds of A. macrorrhizus were extracted, disinfected, and then cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS medium supplemented with 0.3 mg l(- 1) NAA, 0.1 mg l(- 1) KIN, 2 mg l(- 1) ancymidol and 6% sucrose. A percentage of 69.7 +/- 8.0% of the rhizome buds developed shoots, but only 17.4 +/- 7.9% of them rooted. To increase this low rooting rate, the shoots were cultured on Macrorrhizus Rooting Media (MRM) supplemented with three different concentrations of IBA. The highest rooting rate (55.0 +/- 7.9%) was reached when shoots were incubated in MRM-2 consisting of MS medium supplemented with 2 mg l(- 1) IBA and 4% sucrose. The acclimatization rate of the micropropagated plantlets was 90%. The method developed in this study allows the micropropagation of A. macrorrhizus, offering a new option to preserve this almost extinct species.

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Regalado J.J. Carmona-Martin E. Lopez-Granero M. Jimenez-Araujo A. Castro P. y Encina C.L. Micropropagation of Asparagus macrorrhizus a Spanish endemic species in extreme extinction risk. Springer Nature, 2018. https://doi.org/10.1007/s11240-017-1346-9

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Resource type Article
Date of creation 2024-11-05
Date of last revision 2025-02-24
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Metadata identifier 559f07cc-a41f-538d-8d5b-9ba3f016d902
Metadata language Spanish
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High-value dataset category Earth observation and environment
ISO 19115 topic category biota
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Name of the dataset creator Regalado, J.J., Carmona-Martin, E., Lopez-Granero, M., Jimenez-Araujo, A., Castro, P. y Encina, C.L.
Name of the dataset editor Springer Nature
Other identifier DOI: 10.1007/s11240-017-1346-9
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Email of the dataset creator jjrg@eelm.csic.es
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