Metabolomic responses in caged clams, Ruditapes decussatus, exposed to agricultural and urban inputs in a Mediterranean coastal lagoon (Mar Menor, SE Spain)

The Mar Menor is a coastal lagoon affected by the growth of intensive agriculture and urban development in the surrounding area. Large amounts of chemical pollutants from these areas are discharged into El Albujon, a permanent water-course flowing into the lagoon. Biomarkers such as the activity of acetylcholinesterase or antioxidant enzymes have been previously tested in this lagoon demonstrating the presence of neurotoxicity and oxidative stress in clams transplanted in sites affected by the dispersion of the effluent from El Albujon. To complete this traditional toxicology work, a metabolomic profiling of these transplanted organisms has been carried out for the detection of metabolic biomarkers induced by agricultural/urban pollutants. More than 70 metabolites have been quantified using a targeting metabolomics platform based on HPLC-MS. The intracellular metabolic pattern was analyzed by PCA from the digestive gland of clams after 7 and 22 days of transplantation. Results showed a different profile of metabolite between organisms collected from control and exposed sites. At the shorter exposure time, there was an increase in several metabolites in the latter when compared with those from control sites, whereas metabolic profiling at 22 days showed that those metabolites were drastically diminished, with even lower levels than at control sites. These metabolites included: (i) 12 amino acids from the 21 proteogenic and HomoSer, (ii) osmotic protectants such as gamma-butyrobetaine and taurine and (iii) nucleotides such as ITP. Regarding sulfur-containing molecules, taurine could be highlighted as a potential biomarker since its concentration was reduced by more than 30 times after 22 days of exposure, whereas the antioxidant glutathione remained constant in the organisms from both control and exposed sites. Although targeted metabolomics has been shown as an early technique of pollutant effect detection, the two-phase pattern could highlight a more complicated metabolite response to pollutants than classical biomarkers. (C) 2015 Elsevier B.V. All rights reserved.

Data and Resources

This dataset has no data

Metadata
Download
DCAT-AP/NTI-RISP

Basic information
Resource type Text
Date of creation 2024-09-17
Date of last revision 2024-09-17
Show changelog
Metadata identifier 82b4490a-a1ba-5f04-bfb6-ea13d3731d7e
Metadata language Spanish
Themes (NTI-RISP)
High-value dataset category
ISO 19115 topic category biota
Other identifier DOI 10.1016/j.scitotenv.2015.03.136
Keyword URIs
Character encoding UTF-8
Spatial information
INSPIRE identifier ESPMITECOIEPNBMMENOR008
INSPIRE Themes
Geographic identifier Murcia
Coordinate Reference System
Spatial representation Type
Bounding Box
"{\"type\": \"Polygon\", \"coordinates\": [[[-2.34, 37.38], [-0.69, 37.38], [-0.69, 38.76], [-2.34, 38.76], [-2.34, 37.38]]]}"
Spatial resolution of the dataset (m)
Provenance
Lineage statement
Metadata Standard
Conformity
Source dataset
Update frequency
Sources
  1. Science of The Total Environment
  2. vol 524–525
  3. 136-147
Purpose
Process steps
Temporal extent (Start)
Temporal extent (End)
Version notes
Version
Dataset validity
Responsible Party
Name of the dataset creator Campillo, J.A., Sevilla, A., Albentosa, M., Bernal, C., Lozano, A.B., Canovas, M., Leon, V.M.
Name of the dataset maintainer
Identifier of the dataset creator
Email of the dataset creator juan.campillo@ieo.csic.es
Website of the dataset creator
Identifier of the dataset maintainer
Email of the dataset maintainer
Website of the dataset maintainer