Detection of Haplosporidium pinnae from Pinna nobilis Faeces

Pinna nobilis (Linnaeus, 1758) is the largest bivalve endemic to the Mediterranean. It is distributed in a wide range of coastal environments, including estuaries. Pinna nobilis has recently become a critically endangered species (with almost 100% mortality) along the entire Spanish Mediterranean coast. This may be due to coinfections caused by Haplosporidium pinnae and bacterial pathogens such as Mycobacterium spp. We extensively sampled P. nobilis from Mar Menor lagoon (SE Spain), a site where individuals still survive. Using conventional PCR, we found Haplosporidium spp. in 7.1% of mantle and faecal DNA samples in different individuals of P. nobilis. We identified and quantified Haplosporidium pinnae in P. nobilis using Sanger sequencing and qPCR. Faecal H. pinnae detection is non-invasive, unlike biopsies. Therefore, this non-lethal and non-invasive sampling method could contribute to the welfare of living populations, particularly in eutrophic environments, where they are prone to septicaemia. The use of faecal DNA analysis could be a major advance in epidemiology and recovery assessment studies of P. nobilis.

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Lopez-Nunez R. Cortes Melendreras E. Gimenez Casalduero F. Prado P. Lopez-Moya F. y Lopez-Llorca L.V. Detection of Haplosporidium pinnae from Pinna nobilis Faeces. MDPI, 2022. https://doi.org/10.3390/jmse10020276

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Retrieved: 24 Feb 2025 14:22:49
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Resource type Article
Date of creation 2024-11-05
Date of last revision 2025-02-24
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Metadata identifier c70fc739-2f90-59f5-8b6a-13c64b266df4
Metadata language Spanish
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High-value dataset category Earth observation and environment
ISO 19115 topic category biota
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Name of the dataset creator Lopez-Nunez, R., Cortes Melendreras, E., Gimenez Casalduero, F., Prado, P., Lopez-Moya, F. y Lopez-Llorca, L.V.
Name of the dataset editor MDPI
Other identifier DOI: 10.3390/jmse10020276
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Email of the dataset creator rln3@gcloud.ua.es
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