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en Integrated chemical and biological analysis to explain estrogenic potency in bile extracts of red mullet (Mullus barbatus) -
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) en Integrated chemical and biological analysis to explain estrogenic potency in bile extracts of red mullet (Mullus barbatus)
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| 102 | "notes": "A biological screening was performed to establish the | 112 | "notes": "A biological screening was performed to establish the | ||
| 103 | total exposure to estrogenic compounds of red mullet (Mullus barbatus) | 113 | total exposure to estrogenic compounds of red mullet (Mullus barbatus) | ||
| 104 | collected at several sites along the Spanish Mediterranean coast by | 114 | collected at several sites along the Spanish Mediterranean coast by | ||
| 105 | testing male fish bile extracts using the in vitro ER-LUC reporter | 115 | testing male fish bile extracts using the in vitro ER-LUC reporter | ||
| 106 | gene assay. In addition, major metabolites were identified and | 116 | gene assay. In addition, major metabolites were identified and | ||
| 107 | measurements of OH-PAHs (1-naphthol, 9-phenantrol, 9-fluorenol, | 117 | measurements of OH-PAHs (1-naphthol, 9-phenantrol, 9-fluorenol, | ||
| 108 | 1-pyrenol, 1OH-BaP and 3OH-BaP) and alkylphenols (4-n-nonylphenol | 118 | 1-pyrenol, 1OH-BaP and 3OH-BaP) and alkylphenols (4-n-nonylphenol | ||
| 109 | (4-n-NP) and 4-tertoctylphenol (4-tert-OP)) in the same fish bile | 119 | (4-n-NP) and 4-tertoctylphenol (4-tert-OP)) in the same fish bile | ||
| 110 | extracts were taken by gas chromatography-mass spectrometry in | 120 | extracts were taken by gas chromatography-mass spectrometry in | ||
| 111 | electron ionization mode (GC-EI-MS). Relative in vitro estrogenic | 121 | electron ionization mode (GC-EI-MS). Relative in vitro estrogenic | ||
| 112 | potencies of the chemically quantified compounds were also tested. The | 122 | potencies of the chemically quantified compounds were also tested. The | ||
| 113 | highest biliary concentrations of 1-pyrenol, 9-fluorenol and 4-n-NP | 123 | highest biliary concentrations of 1-pyrenol, 9-fluorenol and 4-n-NP | ||
| 114 | were found in fish from Barcelona and from the Mar Menor coastal | 124 | were found in fish from Barcelona and from the Mar Menor coastal | ||
| 115 | lagoon. However, these concentrations can be considered relatively low | 125 | lagoon. However, these concentrations can be considered relatively low | ||
| 116 | compared to values reported in red mullet from other polluted waters | 126 | compared to values reported in red mullet from other polluted waters | ||
| 117 | in the Mediterranean Sea. The contribution of 1-pyrenol, 4-n-NP and | 127 | in the Mediterranean Sea. The contribution of 1-pyrenol, 4-n-NP and | ||
| 118 | 4-tert-OP to the total estrogenic potency measured in male fish bile | 128 | 4-tert-OP to the total estrogenic potency measured in male fish bile | ||
| 119 | was found to be negligible, indicating the presence of other | 129 | was found to be negligible, indicating the presence of other | ||
| 120 | estrogenic compounds in red mullet bile. Estrogenic potency in bile | 130 | estrogenic compounds in red mullet bile. Estrogenic potency in bile | ||
| 121 | from male fish was markedly elevated in Mar Menor lagoon (234.8 +/- | 131 | from male fish was markedly elevated in Mar Menor lagoon (234.8 +/- | ||
| 122 | 5.7 pg E2EQ/mu l), and further research will be necessary to explain | 132 | 5.7 pg E2EQ/mu l), and further research will be necessary to explain | ||
| 123 | whether the presence of natural and synthetic-hormones in the lagoon | 133 | whether the presence of natural and synthetic-hormones in the lagoon | ||
| 124 | contributed to this finding. Values of approximately 15-16 E2EQpg/mg | 134 | contributed to this finding. Values of approximately 15-16 E2EQpg/mg | ||
| 125 | bile can be regarded as preliminary baseline levels of bile | 135 | bile can be regarded as preliminary baseline levels of bile | ||
| 126 | estrogenicity in male red mullet from the western Mediterranean Sea.", | 136 | estrogenicity in male red mullet from the western Mediterranean Sea.", | ||
| 127 | "notes_translated": { | 137 | "notes_translated": { | ||
| 128 | "es": "A biological screening was performed to establish the total | 138 | "es": "A biological screening was performed to establish the total | ||
| 129 | exposure to estrogenic compounds of red mullet (Mullus barbatus) | 139 | exposure to estrogenic compounds of red mullet (Mullus barbatus) | ||
| 130 | collected at several sites along the Spanish Mediterranean coast by | 140 | collected at several sites along the Spanish Mediterranean coast by | ||
| 131 | testing male fish bile extracts using the in vitro ER-LUC reporter | 141 | testing male fish bile extracts using the in vitro ER-LUC reporter | ||
| 132 | gene assay. In addition, major metabolites were identified and | 142 | gene assay. In addition, major metabolites were identified and | ||
| 133 | measurements of OH-PAHs (1-naphthol, 9-phenantrol, 9-fluorenol, | 143 | measurements of OH-PAHs (1-naphthol, 9-phenantrol, 9-fluorenol, | ||
| 134 | 1-pyrenol, 1OH-BaP and 3OH-BaP) and alkylphenols (4-n-nonylphenol | 144 | 1-pyrenol, 1OH-BaP and 3OH-BaP) and alkylphenols (4-n-nonylphenol | ||
| 135 | (4-n-NP) and 4-tertoctylphenol (4-tert-OP)) in the same fish bile | 145 | (4-n-NP) and 4-tertoctylphenol (4-tert-OP)) in the same fish bile | ||
| 136 | extracts were taken by gas chromatography-mass spectrometry in | 146 | extracts were taken by gas chromatography-mass spectrometry in | ||
| 137 | electron ionization mode (GC-EI-MS). Relative in vitro estrogenic | 147 | electron ionization mode (GC-EI-MS). Relative in vitro estrogenic | ||
| 138 | potencies of the chemically quantified compounds were also tested. The | 148 | potencies of the chemically quantified compounds were also tested. The | ||
| 139 | highest biliary concentrations of 1-pyrenol, 9-fluorenol and 4-n-NP | 149 | highest biliary concentrations of 1-pyrenol, 9-fluorenol and 4-n-NP | ||
| 140 | were found in fish from Barcelona and from the Mar Menor coastal | 150 | were found in fish from Barcelona and from the Mar Menor coastal | ||
| 141 | lagoon. However, these concentrations can be considered relatively low | 151 | lagoon. However, these concentrations can be considered relatively low | ||
| 142 | compared to values reported in red mullet from other polluted waters | 152 | compared to values reported in red mullet from other polluted waters | ||
| 143 | in the Mediterranean Sea. The contribution of 1-pyrenol, 4-n-NP and | 153 | in the Mediterranean Sea. The contribution of 1-pyrenol, 4-n-NP and | ||
| 144 | 4-tert-OP to the total estrogenic potency measured in male fish bile | 154 | 4-tert-OP to the total estrogenic potency measured in male fish bile | ||
| 145 | was found to be negligible, indicating the presence of other | 155 | was found to be negligible, indicating the presence of other | ||
| 146 | estrogenic compounds in red mullet bile. Estrogenic potency in bile | 156 | estrogenic compounds in red mullet bile. Estrogenic potency in bile | ||
| 147 | from male fish was markedly elevated in Mar Menor lagoon (234.8 +/- | 157 | from male fish was markedly elevated in Mar Menor lagoon (234.8 +/- | ||
| 148 | 5.7 pg E2EQ/mu l), and further research will be necessary to explain | 158 | 5.7 pg E2EQ/mu l), and further research will be necessary to explain | ||
| 149 | whether the presence of natural and synthetic-hormones in the lagoon | 159 | whether the presence of natural and synthetic-hormones in the lagoon | ||
| 150 | contributed to this finding. Values of approximately 15-16 E2EQpg/mg | 160 | contributed to this finding. Values of approximately 15-16 E2EQpg/mg | ||
| 151 | bile can be regarded as preliminary baseline levels of bile | 161 | bile can be regarded as preliminary baseline levels of bile | ||
| 152 | estrogenicity in male red mullet from the western Mediterranean Sea." | 162 | estrogenicity in male red mullet from the western Mediterranean Sea." | ||
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