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) en Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk
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| 94 | "notes": "Asparagus macrorrhizus: is a new species, which has been | 104 | "notes": "Asparagus macrorrhizus: is a new species, which has been | ||
| 95 | recently described. It is limited to the area surrounding the \"Mar | 105 | recently described. It is limited to the area surrounding the \"Mar | ||
| 96 | Menor\" lagoon, in Murcia (Spain), and is the only \"Critically | 106 | Menor\" lagoon, in Murcia (Spain), and is the only \"Critically | ||
| 97 | Endangered\" species of the genus Asparagus. Despite being protected, | 107 | Endangered\" species of the genus Asparagus. Despite being protected, | ||
| 98 | the number of plants has decreased in the last years due to the | 108 | the number of plants has decreased in the last years due to the | ||
| 99 | urbanization of its natural habitat. This species is a valuable | 109 | urbanization of its natural habitat. This species is a valuable | ||
| 100 | genetic resource for asparagus breeding because of its special | 110 | genetic resource for asparagus breeding because of its special | ||
| 101 | characteristics. So, the development of a micropropagation protocol is | 111 | characteristics. So, the development of a micropropagation protocol is | ||
| 102 | crucial to its conservation and use in breeding programs. The | 112 | crucial to its conservation and use in breeding programs. The | ||
| 103 | micropropagation protocol from asparagus rhizome buds previously | 113 | micropropagation protocol from asparagus rhizome buds previously | ||
| 104 | developed by our research group has been adapted for A. macrorrhizus. | 114 | developed by our research group has been adapted for A. macrorrhizus. | ||
| 105 | Rhizome buds of A. macrorrhizus were extracted, disinfected, and then | 115 | Rhizome buds of A. macrorrhizus were extracted, disinfected, and then | ||
| 106 | cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS | 116 | cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS | ||
| 107 | medium supplemented with 0.3 mg l(- 1) NAA, 0.1 mg l(- 1) KIN, 2 mg | 117 | medium supplemented with 0.3 mg l(- 1) NAA, 0.1 mg l(- 1) KIN, 2 mg | ||
| 108 | l(- 1) ancymidol and 6% sucrose. A percentage of 69.7 +/- 8.0% of the | 118 | l(- 1) ancymidol and 6% sucrose. A percentage of 69.7 +/- 8.0% of the | ||
| 109 | rhizome buds developed shoots, but only 17.4 +/- 7.9% of them rooted. | 119 | rhizome buds developed shoots, but only 17.4 +/- 7.9% of them rooted. | ||
| 110 | To increase this low rooting rate, the shoots were cultured on | 120 | To increase this low rooting rate, the shoots were cultured on | ||
| 111 | Macrorrhizus Rooting Media (MRM) supplemented with three different | 121 | Macrorrhizus Rooting Media (MRM) supplemented with three different | ||
| 112 | concentrations of IBA. The highest rooting rate (55.0 +/- 7.9%) was | 122 | concentrations of IBA. The highest rooting rate (55.0 +/- 7.9%) was | ||
| 113 | reached when shoots were incubated in MRM-2 consisting of MS medium | 123 | reached when shoots were incubated in MRM-2 consisting of MS medium | ||
| 114 | supplemented with 2 mg l(- 1) IBA and 4% sucrose. The acclimatization | 124 | supplemented with 2 mg l(- 1) IBA and 4% sucrose. The acclimatization | ||
| 115 | rate of the micropropagated plantlets was 90%. The method developed in | 125 | rate of the micropropagated plantlets was 90%. The method developed in | ||
| 116 | this study allows the micropropagation of A. macrorrhizus, offering a | 126 | this study allows the micropropagation of A. macrorrhizus, offering a | ||
| 117 | new option to preserve this almost extinct species.", | 127 | new option to preserve this almost extinct species.", | ||
| 118 | "notes_translated": { | 128 | "notes_translated": { | ||
| 119 | "es": "Asparagus macrorrhizus: is a new species, which has been | 129 | "es": "Asparagus macrorrhizus: is a new species, which has been | ||
| 120 | recently described. It is limited to the area surrounding the \"Mar | 130 | recently described. It is limited to the area surrounding the \"Mar | ||
| 121 | Menor\" lagoon, in Murcia (Spain), and is the only \"Critically | 131 | Menor\" lagoon, in Murcia (Spain), and is the only \"Critically | ||
| 122 | Endangered\" species of the genus Asparagus. Despite being protected, | 132 | Endangered\" species of the genus Asparagus. Despite being protected, | ||
| 123 | the number of plants has decreased in the last years due to the | 133 | the number of plants has decreased in the last years due to the | ||
| 124 | urbanization of its natural habitat. This species is a valuable | 134 | urbanization of its natural habitat. This species is a valuable | ||
| 125 | genetic resource for asparagus breeding because of its special | 135 | genetic resource for asparagus breeding because of its special | ||
| 126 | characteristics. So, the development of a micropropagation protocol is | 136 | characteristics. So, the development of a micropropagation protocol is | ||
| 127 | crucial to its conservation and use in breeding programs. The | 137 | crucial to its conservation and use in breeding programs. The | ||
| 128 | micropropagation protocol from asparagus rhizome buds previously | 138 | micropropagation protocol from asparagus rhizome buds previously | ||
| 129 | developed by our research group has been adapted for A. macrorrhizus. | 139 | developed by our research group has been adapted for A. macrorrhizus. | ||
| 130 | Rhizome buds of A. macrorrhizus were extracted, disinfected, and then | 140 | Rhizome buds of A. macrorrhizus were extracted, disinfected, and then | ||
| 131 | cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS | 141 | cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS | ||
| 132 | medium supplemented with 0.3 mg l(- 1) NAA, 0.1 mg l(- 1) KIN, 2 mg | 142 | medium supplemented with 0.3 mg l(- 1) NAA, 0.1 mg l(- 1) KIN, 2 mg | ||
| 133 | l(- 1) ancymidol and 6% sucrose. A percentage of 69.7 +/- 8.0% of the | 143 | l(- 1) ancymidol and 6% sucrose. A percentage of 69.7 +/- 8.0% of the | ||
| 134 | rhizome buds developed shoots, but only 17.4 +/- 7.9% of them rooted. | 144 | rhizome buds developed shoots, but only 17.4 +/- 7.9% of them rooted. | ||
| 135 | To increase this low rooting rate, the shoots were cultured on | 145 | To increase this low rooting rate, the shoots were cultured on | ||
| 136 | Macrorrhizus Rooting Media (MRM) supplemented with three different | 146 | Macrorrhizus Rooting Media (MRM) supplemented with three different | ||
| 137 | concentrations of IBA. The highest rooting rate (55.0 +/- 7.9%) was | 147 | concentrations of IBA. The highest rooting rate (55.0 +/- 7.9%) was | ||
| 138 | reached when shoots were incubated in MRM-2 consisting of MS medium | 148 | reached when shoots were incubated in MRM-2 consisting of MS medium | ||
| 139 | supplemented with 2 mg l(- 1) IBA and 4% sucrose. The acclimatization | 149 | supplemented with 2 mg l(- 1) IBA and 4% sucrose. The acclimatization | ||
| 140 | rate of the micropropagated plantlets was 90%. The method developed in | 150 | rate of the micropropagated plantlets was 90%. The method developed in | ||
| 141 | this study allows the micropropagation of A. macrorrhizus, offering a | 151 | this study allows the micropropagation of A. macrorrhizus, offering a | ||
| 142 | new option to preserve this almost extinct species." | 152 | new option to preserve this almost extinct species." | ||
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| 254 | "study_variables": { | 264 | "study_variables": { | ||
| 255 | "es": "" | 265 | "es": "" | ||
| 256 | }, | 266 | }, | ||
| 257 | "tag_uri": [ | 267 | "tag_uri": [ | ||
| 258 | 268 | ||||
| 259 | tp://inspire.ec.europa.eu/metadata-codelist/TopicCategory/environment" | 269 | tp://inspire.ec.europa.eu/metadata-codelist/TopicCategory/environment" | ||
| 260 | ], | 270 | ], | ||
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| 264 | "id": "9532991a-ec15-4a7c-b7f9-8ec12fcd335d", | 274 | "id": "9532991a-ec15-4a7c-b7f9-8ec12fcd335d", | ||
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| 270 | "display_name": "cultivos", | 280 | "display_name": "cultivos", | ||
| 271 | "id": "548d273f-b76d-4cd2-9f9a-4e12caea3458", | 281 | "id": "548d273f-b76d-4cd2-9f9a-4e12caea3458", | ||
| 272 | "name": "cultivos", | 282 | "name": "cultivos", | ||
| 273 | "state": "active", | 283 | "state": "active", | ||
| 274 | "vocabulary_id": null | 284 | "vocabulary_id": null | ||
| 275 | }, | 285 | }, | ||
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| 277 | "display_name": "diversidad_genetica", | 287 | "display_name": "diversidad_genetica", | ||
| 278 | "id": "36b1105b-eac3-4355-a30c-57c1a0a3e973", | 288 | "id": "36b1105b-eac3-4355-a30c-57c1a0a3e973", | ||
| 279 | "name": "diversidad_genetica", | 289 | "name": "diversidad_genetica", | ||
| 280 | "state": "active", | 290 | "state": "active", | ||
| 281 | "vocabulary_id": null | 291 | "vocabulary_id": null | ||
| 282 | }, | 292 | }, | ||
| 283 | { | 293 | { | ||
| 284 | "display_name": "gestion_sostenible", | 294 | "display_name": "gestion_sostenible", | ||
| 285 | "id": "ab71cc3e-3c99-4f3f-a20f-d4e8016c3b55", | 295 | "id": "ab71cc3e-3c99-4f3f-a20f-d4e8016c3b55", | ||
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| 288 | "vocabulary_id": null | 298 | "vocabulary_id": null | ||
| 289 | }, | 299 | }, | ||
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| 292 | "id": "004084d1-68d8-46ca-842a-c10278540bc9", | 302 | "id": "004084d1-68d8-46ca-842a-c10278540bc9", | ||
| 293 | "name": "marino", | 303 | "name": "marino", | ||
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| 296 | } | 306 | } | ||
| 297 | ], | 307 | ], | ||
| 298 | "thematic_area": [ | 308 | "thematic_area": [ | ||
| 299 | "espacios_protegidos" | 309 | "espacios_protegidos" | ||
| 300 | ], | 310 | ], | ||
| 301 | "theme_es": [ | 311 | "theme_es": [ | ||
| 302 | "http://datos.gob.es/kos/sector-publico/sector/medio-ambiente" | 312 | "http://datos.gob.es/kos/sector-publico/sector/medio-ambiente" | ||
| 303 | ], | 313 | ], | ||
| 304 | "title": "Micropropagation of Asparagus macrorrhizus, a Spanish | 314 | "title": "Micropropagation of Asparagus macrorrhizus, a Spanish | ||
| 305 | endemic species in extreme extinction risk", | 315 | endemic species in extreme extinction risk", | ||
| 306 | "title_translated": { | 316 | "title_translated": { | ||
| 307 | "es": "Micropropagation of Asparagus macrorrhizus, a Spanish | 317 | "es": "Micropropagation of Asparagus macrorrhizus, a Spanish | ||
| 308 | endemic species in extreme extinction risk" | 318 | endemic species in extreme extinction risk" | ||
| 309 | }, | 319 | }, | ||
| 310 | "topic": | 320 | "topic": | ||
| 311 | "http://inspire.ec.europa.eu/metadata-codelist/TopicCategory/biota", | 321 | "http://inspire.ec.europa.eu/metadata-codelist/TopicCategory/biota", | ||
| 312 | "type": "dataset", | 322 | "type": "dataset", | ||
| 313 | "url": | 323 | "url": | ||
| 314 | //iepnb.es:443/catalogo/dataset/559f07cc-a41f-538d-8d5b-9ba3f016d902", | 324 | //iepnb.es:443/catalogo/dataset/559f07cc-a41f-538d-8d5b-9ba3f016d902", | ||
| 315 | "version_notes": { | 325 | "version_notes": { | ||
| 316 | "es": "" | 326 | "es": "" | ||
| 317 | } | 327 | } | ||
| 318 | } | 328 | } |