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) en Benthic Diatoms from the Hypersaline Mar Menor coastal lagoon: Taxonomy, phylogeny and responses to environmental changes
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| 86 | "notes": "Introduction and objectives Diatoms are the most abundant | 96 | "notes": "Introduction and objectives Diatoms are the most abundant | ||
| 87 | group within benthic microalgae that form biofilms in aquatic systems. | 97 | group within benthic microalgae that form biofilms in aquatic systems. | ||
| 88 | These communities play a key role in the functioning of shallow | 98 | These communities play a key role in the functioning of shallow | ||
| 89 | coastal systems, such as lagoons and estuaries, at the primary | 99 | coastal systems, such as lagoons and estuaries, at the primary | ||
| 90 | production level and in biogeochemical cycles, and are considered the | 100 | production level and in biogeochemical cycles, and are considered the | ||
| 91 | basis of the food chain. They also constitute one of the first | 101 | basis of the food chain. They also constitute one of the first | ||
| 92 | compartments that react to any environmental variation or type of | 102 | compartments that react to any environmental variation or type of | ||
| 93 | impact. In the marine environment, information on distribution, | 103 | impact. In the marine environment, information on distribution, | ||
| 94 | ecology and taxonomy, and the use of benthic diatoms as biological | 104 | ecology and taxonomy, and the use of benthic diatoms as biological | ||
| 95 | indicators of environmental changes, are scarce. The present assay was | 105 | indicators of environmental changes, are scarce. The present assay was | ||
| 96 | carried out in the coastal Mar Menor lagoon (SE Spain), where previous | 106 | carried out in the coastal Mar Menor lagoon (SE Spain), where previous | ||
| 97 | floristic information practically cannot be found. The lagoon is | 107 | floristic information practically cannot be found. The lagoon is | ||
| 98 | subject to multiple anthropogenic impacts, including contributions of | 108 | subject to multiple anthropogenic impacts, including contributions of | ||
| 99 | metals and nutrients loads. Populations may be strongly affected by | 109 | metals and nutrients loads. Populations may be strongly affected by | ||
| 100 | the increase in temperature associated with global climate change. | 110 | the increase in temperature associated with global climate change. | ||
| 101 | Therefore, it is an ideal study area to investigate the relationships | 111 | Therefore, it is an ideal study area to investigate the relationships | ||
| 102 | of species and communities with environmental gradients, and it may | 112 | of species and communities with environmental gradients, and it may | ||
| 103 | help to understand the biotic and abiotic factors that structure | 113 | help to understand the biotic and abiotic factors that structure | ||
| 104 | benthic diatom communities. The general objective of this thesis is to | 114 | benthic diatom communities. The general objective of this thesis is to | ||
| 105 | improve knowledge about the ecology and taxonomy of the benthic diatom | 115 | improve knowledge about the ecology and taxonomy of the benthic diatom | ||
| 106 | communities in the coastal Mar Menor lagoon and to evaluate their | 116 | communities in the coastal Mar Menor lagoon and to evaluate their | ||
| 107 | potential as biological indicators of the multiple stressors that | 117 | potential as biological indicators of the multiple stressors that | ||
| 108 | affect and threaten the lagoon. Any work conducted on bioindicators is | 118 | affect and threaten the lagoon. Any work conducted on bioindicators is | ||
| 109 | based on good floristic knowledge, but this information is lacking in | 119 | based on good floristic knowledge, but this information is lacking in | ||
| 110 | Mar Menor. For this reason, the following specific objectives were | 120 | Mar Menor. For this reason, the following specific objectives were | ||
| 111 | identified: i) to morphologically, ecologically and phylogenetically | 121 | identified: i) to morphologically, ecologically and phylogenetically | ||
| 112 | study the genus Hyalosynedra; ii) investigate the composition and | 122 | study the genus Hyalosynedra; ii) investigate the composition and | ||
| 113 | abundance of the species of the genus Licmophora in the lagoon, and | 123 | abundance of the species of the genus Licmophora in the lagoon, and | ||
| 114 | its phylogenetic relationships; iii) evaluate the combined effect of | 124 | its phylogenetic relationships; iii) evaluate the combined effect of | ||
| 115 | temperature and the stoichiometric N:P ratio of water on populations | 125 | temperature and the stoichiometric N:P ratio of water on populations | ||
| 116 | of the stalked marine diatom Licmophora colosalis, and to test whether | 126 | of the stalked marine diatom Licmophora colosalis, and to test whether | ||
| 117 | overproduction of stalks could serve as an indicator of nutrient | 127 | overproduction of stalks could serve as an indicator of nutrient | ||
| 118 | contamination; iv) study the composition, structure and biological | 128 | contamination; iv) study the composition, structure and biological | ||
| 119 | traits of the species of two benthic diatom communities influenced | 129 | traits of the species of two benthic diatom communities influenced | ||
| 120 | differently by the chronic contamination of metals and nutrients, and | 130 | differently by the chronic contamination of metals and nutrients, and | ||
| 121 | investigate their responses to the sporadic exposure of both factors. | 131 | investigate their responses to the sporadic exposure of both factors. | ||
| 122 | These objectives aim to evaluate the suitability of these communities | 132 | These objectives aim to evaluate the suitability of these communities | ||
| 123 | as a tool to environmentally monitor the lagoon. Methodology Floristic | 133 | as a tool to environmentally monitor the lagoon. Methodology Floristic | ||
| 124 | works include morphological descriptions obtained by optical | 134 | works include morphological descriptions obtained by optical | ||
| 125 | microscopy and scanning electron microscopy, and by molecular analyses | 135 | microscopy and scanning electron microscopy, and by molecular analyses | ||
| 126 | using cell cultures, extraction of genetic material, sequencing and | 136 | using cell cultures, extraction of genetic material, sequencing and | ||
| 127 | phylogenetic analyses of sequences. Laboratory bioassays consist of | 137 | phylogenetic analyses of sequences. Laboratory bioassays consist of | ||
| 128 | applying different temperatures and nutrient treatments to evaluate | 138 | applying different temperatures and nutrient treatments to evaluate | ||
| 129 | the effect on population dynamics (growth measures, cell size, | 139 | the effect on population dynamics (growth measures, cell size, | ||
| 130 | mortality), stalks production (by image analyses) and carbohydrates | 140 | mortality), stalks production (by image analyses) and carbohydrates | ||
| 131 | (chemical analyses) of Licmophroa colosalis. The field experiment | 141 | (chemical analyses) of Licmophroa colosalis. The field experiment | ||
| 132 | involves applying different treatments of metals and nutrients | 142 | involves applying different treatments of metals and nutrients | ||
| 133 | individually and combined.", | 143 | individually and combined.", | ||
| 134 | "notes_translated": { | 144 | "notes_translated": { | ||
| 135 | "es": "Introduction and objectives Diatoms are the most abundant | 145 | "es": "Introduction and objectives Diatoms are the most abundant | ||
| 136 | group within benthic microalgae that form biofilms in aquatic systems. | 146 | group within benthic microalgae that form biofilms in aquatic systems. | ||
| 137 | These communities play a key role in the functioning of shallow | 147 | These communities play a key role in the functioning of shallow | ||
| 138 | coastal systems, such as lagoons and estuaries, at the primary | 148 | coastal systems, such as lagoons and estuaries, at the primary | ||
| 139 | production level and in biogeochemical cycles, and are considered the | 149 | production level and in biogeochemical cycles, and are considered the | ||
| 140 | basis of the food chain. They also constitute one of the first | 150 | basis of the food chain. They also constitute one of the first | ||
| 141 | compartments that react to any environmental variation or type of | 151 | compartments that react to any environmental variation or type of | ||
| 142 | impact. In the marine environment, information on distribution, | 152 | impact. In the marine environment, information on distribution, | ||
| 143 | ecology and taxonomy, and the use of benthic diatoms as biological | 153 | ecology and taxonomy, and the use of benthic diatoms as biological | ||
| 144 | indicators of environmental changes, are scarce. The present assay was | 154 | indicators of environmental changes, are scarce. The present assay was | ||
| 145 | carried out in the coastal Mar Menor lagoon (SE Spain), where previous | 155 | carried out in the coastal Mar Menor lagoon (SE Spain), where previous | ||
| 146 | floristic information practically cannot be found. The lagoon is | 156 | floristic information practically cannot be found. The lagoon is | ||
| 147 | subject to multiple anthropogenic impacts, including contributions of | 157 | subject to multiple anthropogenic impacts, including contributions of | ||
| 148 | metals and nutrients loads. Populations may be strongly affected by | 158 | metals and nutrients loads. Populations may be strongly affected by | ||
| 149 | the increase in temperature associated with global climate change. | 159 | the increase in temperature associated with global climate change. | ||
| 150 | Therefore, it is an ideal study area to investigate the relationships | 160 | Therefore, it is an ideal study area to investigate the relationships | ||
| 151 | of species and communities with environmental gradients, and it may | 161 | of species and communities with environmental gradients, and it may | ||
| 152 | help to understand the biotic and abiotic factors that structure | 162 | help to understand the biotic and abiotic factors that structure | ||
| 153 | benthic diatom communities. The general objective of this thesis is to | 163 | benthic diatom communities. The general objective of this thesis is to | ||
| 154 | improve knowledge about the ecology and taxonomy of the benthic diatom | 164 | improve knowledge about the ecology and taxonomy of the benthic diatom | ||
| 155 | communities in the coastal Mar Menor lagoon and to evaluate their | 165 | communities in the coastal Mar Menor lagoon and to evaluate their | ||
| 156 | potential as biological indicators of the multiple stressors that | 166 | potential as biological indicators of the multiple stressors that | ||
| 157 | affect and threaten the lagoon. Any work conducted on bioindicators is | 167 | affect and threaten the lagoon. Any work conducted on bioindicators is | ||
| 158 | based on good floristic knowledge, but this information is lacking in | 168 | based on good floristic knowledge, but this information is lacking in | ||
| 159 | Mar Menor. For this reason, the following specific objectives were | 169 | Mar Menor. For this reason, the following specific objectives were | ||
| 160 | identified: i) to morphologically, ecologically and phylogenetically | 170 | identified: i) to morphologically, ecologically and phylogenetically | ||
| 161 | study the genus Hyalosynedra; ii) investigate the composition and | 171 | study the genus Hyalosynedra; ii) investigate the composition and | ||
| 162 | abundance of the species of the genus Licmophora in the lagoon, and | 172 | abundance of the species of the genus Licmophora in the lagoon, and | ||
| 163 | its phylogenetic relationships; iii) evaluate the combined effect of | 173 | its phylogenetic relationships; iii) evaluate the combined effect of | ||
| 164 | temperature and the stoichiometric N:P ratio of water on populations | 174 | temperature and the stoichiometric N:P ratio of water on populations | ||
| 165 | of the stalked marine diatom Licmophora colosalis, and to test whether | 175 | of the stalked marine diatom Licmophora colosalis, and to test whether | ||
| 166 | overproduction of stalks could serve as an indicator of nutrient | 176 | overproduction of stalks could serve as an indicator of nutrient | ||
| 167 | contamination; iv) study the composition, structure and biological | 177 | contamination; iv) study the composition, structure and biological | ||
| 168 | traits of the species of two benthic diatom communities influenced | 178 | traits of the species of two benthic diatom communities influenced | ||
| 169 | differently by the chronic contamination of metals and nutrients, and | 179 | differently by the chronic contamination of metals and nutrients, and | ||
| 170 | investigate their responses to the sporadic exposure of both factors. | 180 | investigate their responses to the sporadic exposure of both factors. | ||
| 171 | These objectives aim to evaluate the suitability of these communities | 181 | These objectives aim to evaluate the suitability of these communities | ||
| 172 | as a tool to environmentally monitor the lagoon. Methodology Floristic | 182 | as a tool to environmentally monitor the lagoon. Methodology Floristic | ||
| 173 | works include morphological descriptions obtained by optical | 183 | works include morphological descriptions obtained by optical | ||
| 174 | microscopy and scanning electron microscopy, and by molecular analyses | 184 | microscopy and scanning electron microscopy, and by molecular analyses | ||
| 175 | using cell cultures, extraction of genetic material, sequencing and | 185 | using cell cultures, extraction of genetic material, sequencing and | ||
| 176 | phylogenetic analyses of sequences. Laboratory bioassays consist of | 186 | phylogenetic analyses of sequences. Laboratory bioassays consist of | ||
| 177 | applying different temperatures and nutrient treatments to evaluate | 187 | applying different temperatures and nutrient treatments to evaluate | ||
| 178 | the effect on population dynamics (growth measures, cell size, | 188 | the effect on population dynamics (growth measures, cell size, | ||
| 179 | mortality), stalks production (by image analyses) and carbohydrates | 189 | mortality), stalks production (by image analyses) and carbohydrates | ||
| 180 | (chemical analyses) of Licmophroa colosalis. The field experiment | 190 | (chemical analyses) of Licmophroa colosalis. The field experiment | ||
| 181 | involves applying different treatments of metals and nutrients | 191 | involves applying different treatments of metals and nutrients | ||
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