Carnivores are one of the most affected taxa by roadkill of their high mobility searching for food, mate or territory. We compared 85 polecat roadkills in highways with 73 casualties on conventional roads. Roadkill points were characterized by a higher abundance of polecat main prey in both road types. On highways occurred in wider sections, on conventional roads were associated with areas of remnant natural vegetation. Our results highlight the need for different roadkill mitigation strategies depending on the road characteristics.