Recording wildlife mortality on railways is challenging as they have narrow corridors and lower accessibility. To improve mitigation measures, surveys must be systematic and their frequency depending on the targeted species traits and biology. To obtain unbiased estimates in diverse contexts, the data should be corrected using mortality estimators. Mitigation measures must avoid that animals remain on the tracks, as trains cannot be instantly stopped. Box culverts, amphibian tunnels, and under- or overpasses allow a safe crossing, whereas exclusion fences, olfactory repellents, sound signals and sound barriers prevent the crossing of railways. Habitat management in railway verges improves the animal capability to evade trains.