@prefix adms: . @prefix cnt: . @prefix dcat: . @prefix dcatap: . @prefix dct: . @prefix eli: . @prefix foaf: . @prefix gsp: . @prefix locn: . @prefix skos: . @prefix vcard: . @prefix xsd: . a dcat:Dataset ; dcatap:applicableLegislation ; dcatap:hvdCategory ; dct:accessRights ; dct:conformsTo ; dct:creator [ a foaf:Agent ; dct:identifier "E05068001" ; dct:type ; foaf:mbox ; foaf:name "Regalado, J.J., Carmona-Martin, E., Lopez-Granero, M., Jimenez-Araujo, A., Castro, P. y Encina, C.L."@en ] ; dct:description "Asparagus macrorrhizus: is a new species, which has been recently described. It is limited to the area surrounding the \"Mar Menor\" lagoon, in Murcia (Spain), and is the only \"Critically Endangered\" species of the genus Asparagus. Despite being protected, the number of plants has decreased in the last years due to the urbanization of its natural habitat. This species is a valuable genetic resource for asparagus breeding because of its special characteristics. So, the development of a micropropagation protocol is crucial to its conservation and use in breeding programs. The micropropagation protocol from asparagus rhizome buds previously developed by our research group has been adapted for A. macrorrhizus. Rhizome buds of A. macrorrhizus were extracted, disinfected, and then cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS medium supplemented with 0.3 mg l(- 1) NAA, 0.1 mg l(- 1) KIN, 2 mg l(- 1) ancymidol and 6% sucrose. A percentage of 69.7 +/- 8.0% of the rhizome buds developed shoots, but only 17.4 +/- 7.9% of them rooted. To increase this low rooting rate, the shoots were cultured on Macrorrhizus Rooting Media (MRM) supplemented with three different concentrations of IBA. The highest rooting rate (55.0 +/- 7.9%) was reached when shoots were incubated in MRM-2 consisting of MS medium supplemented with 2 mg l(- 1) IBA and 4% sucrose. The acclimatization rate of the micropropagated plantlets was 90%. The method developed in this study allows the micropropagation of A. macrorrhizus, offering a new option to preserve this almost extinct species."@en, "Asparagus macrorrhizus: is a new species, which has been recently described. It is limited to the area surrounding the \"Mar Menor\" lagoon, in Murcia (Spain), and is the only \"Critically Endangered\" species of the genus Asparagus. Despite being protected, the number of plants has decreased in the last years due to the urbanization of its natural habitat. This species is a valuable genetic resource for asparagus breeding because of its special characteristics. So, the development of a micropropagation protocol is crucial to its conservation and use in breeding programs. The micropropagation protocol from asparagus rhizome buds previously developed by our research group has been adapted for A. macrorrhizus. Rhizome buds of A. macrorrhizus were extracted, disinfected, and then cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS medium supplemented with 0.3 mg l(- 1) NAA, 0.1 mg l(- 1) KIN, 2 mg l(- 1) ancymidol and 6% sucrose. A percentage of 69.7 +/- 8.0% of the rhizome buds developed shoots, but only 17.4 +/- 7.9% of them rooted. To increase this low rooting rate, the shoots were cultured on Macrorrhizus Rooting Media (MRM) supplemented with three different concentrations of IBA. The highest rooting rate (55.0 +/- 7.9%) was reached when shoots were incubated in MRM-2 consisting of MS medium supplemented with 2 mg l(- 1) IBA and 4% sucrose. The acclimatization rate of the micropropagated plantlets was 90%. The method developed in this study allows the micropropagation of A. macrorrhizus, offering a new option to preserve this almost extinct species."@es ; dct:identifier "559f07cc-a41f-538d-8d5b-9ba3f016d902" ; dct:issued "2024-11-05T00:00:00+00:00"^^xsd:dateTime ; dct:language ; dct:license ; dct:modified "2026-06-25T00:00:00+00:00"^^xsd:dateTime ; dct:publisher ; dct:spatial ; dct:title "Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk"@en, "Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk"@es ; dct:type ; adms:identifier "DOI: 10.1007/s11240-017-1346-9" ; adms:status ; dcat:contactPoint ; dcat:distribution ; dcat:keyword "amenazas"@en ; dcat:landingPage ; dcat:theme , . a dct:Location ; skos:prefLabel "Región de Murcia" ; dcat:bbox "POLYGON ((-2.3400 37.3800, -0.6900 37.3800, -0.6900 38.7600, -2.3400 38.7600, -2.3400 37.3800))"^^gsp:wktLiteral ; dcat:centroid "POINT (-1.5150 38.0700)"^^gsp:wktLiteral ; locn:geometry "POLYGON ((-2.3400 37.3800, -0.6900 37.3800, -0.6900 38.7600, -2.3400 38.7600, -2.3400 37.3800))"^^gsp:wktLiteral . a dct:LinguisticSystem . a dct:LinguisticSystem . a foaf:Agent ; dct:type ; vcard:hasEmail ; foaf:homepage ; foaf:name "Área de Banco de Datos de la Naturaleza. Dirección General Biodiversidad, Bosques y Desertificación. Ministerio para la Transición Ecológica y el Reto Demográfico"@en . a foaf:Document . a dcat:Distribution ; dcatap:applicableLegislation ; dcatap:availability ; dct:accessRights ; dct:format ; dct:issued "2018-03-01T00:00:00+00:00"^^xsd:dateTime ; dct:language ; dct:license ; dct:modified "2026-06-25T00:00:00+00:00"^^xsd:dateTime ; dct:title "Distribución HTML"@en, "Distribución HTML"@es ; cnt:characterEncoding "UTF-8" ; dcat:accessURL ; dcat:mediaType . a vcard:Kind ; vcard:fn "Organismo publicador del Catálogo"@en ; vcard:hasEmail ; vcard:hasURL ; vcard:role . a dct:Standard . a eli:LegalResource . dct:type .