This study was to investigate the Genetic characteristic of ragged sea hare Bursatella leachii found in Indian water is similar to the species found in Mar Menor (SE Spain) water since pathways/ vector of species introduced into the world wide by the way of transportation activity (shipping activity such as ballast water, sedimentation, anchoring & fouling), aquarium trading (trading of live marine species) and aquaculture activity. The species found in Pulicate Lake, India is genetically matching with the species found in SE Spain water species by 99%. The genetic homogeneity pattern of Bursatella leachii found in Mar Menor (SE Spain) and Pulicat Lake in India is the same species available here.